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KMID : 0363420090230010001
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Journal of Korean Oriental Pediatrics
2009 Volume.23 No. 1 p.1 ~ p.22
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The Effect of Li Zhong Tang on the Suppression of Th2 Differentiation by Response in IgE Hyperproduction and Atopic Dermatitis-like Skin Lesions Induced NC/Nga Mouse
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Seo Hui-Yeon
Han Jae-Kyung
Kim Yun-Hee
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Abstract
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Objectives: The purpose of this study is to investigate the effect of Li Zhong Tang(LZH-T) on atopic dermatitis by using NC/Nga atopic dermatitis mouse.
Methods: First, in vitro, we isolated B cells from NC/Nga mouse which induced atopic dermatitis-like skin for 18 weeks. We analyzed FACS by intracellular staining of , GATA-3+ and also analyzed cytokines by using real-time PCR. Secondly, in vivo, after administration of LZH-T to the 12 weeks old mouse with atopic dermatitis. We analyzed serum IgE, level and observed the changes of activated cell.
Results: In vitro, LZH-T decreased the levels of CD4+/ and increased the levels of CD4+/GATA3+. In vivo, serum IgE levels were decreased and levels were increased in LZH-T group compared to the control group. In PBMCs, the percentage of activated cell - granulocytes, CD3+, CD3+CD4+, B220+CD23+, and CCR3+ were decreased and CD19+, CD3+CD8+ were increased in LZH-T group compared to the control group.
Conclusions: This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.
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KEYWORD
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Atopic dermatitis, Li Zhong Tang, NC/Nga mouse
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